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Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae


Citation

Awla, Hayman Kakakhan and Kadir, Jugah and Othman, Radziah and Rashid, Tavga Sulaiman and Wong, Mui Yun (2016) Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae. American Journal of Plant Sciences, 7 (7). art. no. 66540. pp. 1077-1085. ISSN 2158-2742; ESSN: 2158-2750

Abstract / Synopsis

A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was to determine the antifungal activity of Streptomyces sp. isolate UPMRS4 extracts against P. oryzae and to identify bioactive antifungal compounds produced by UPMRS4. Various solvents were used for extraction of antifungal compounds and well diffusion method was used to determine the antifungal activity of the extracts. The ethyl acetate extract demonstrated the highest activity against mycelial growth of P. oryzae, with an effective inhibitory concentration (EIC) of 1.562 μg/ml significantly higher compared to that of chloroform, diethyl ether, methanol, acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses,compounds with antifungal activity were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl); Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl); ergotamine; amicomacin; fungichromin; rapamycin and N-Acetyl-D, L-phenylalanine. These compounds had good general antifungal activity and might have potential future agricultural applications.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.4236/ajps.2016.77103
Publisher: Scientific Research Publishing
Keywords: GCMS; LCMS/MS; Antifungal; Streptomyces sp.; Rice blast
Depositing User: Nida Hidayati Ghazali
Date Deposited: 22 Jan 2018 18:16
Last Modified: 22 Jan 2018 18:16
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4236/ajps.2016.77103
URI: http://psasir.upm.edu.my/id/eprint/55200
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