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Binding energy and biophysical properties of ionic liquid-DNA complex: understanding the role of hydrophobic interactions


Citation

Jumbri, Khairulazhar and Ahmad, Haslina and Abdul Malek, Emilia and Abdul Rahman, Mohd Basyaruddin (2016) Binding energy and biophysical properties of ionic liquid-DNA complex: understanding the role of hydrophobic interactions. Journal of Molecular Liquids, 223. pp. 1197-1203. ISSN 0167-7322; ESSN: 1873-3166

Abstract

The properties of DNA in ionic liquids (ILs) have been well studied. However, it is not widely known about the quantitative analysis of ILs such as the binding profile, which could offer a detailed insight of their binding characteristic. In this study, the binding energy of alkylimidazolium-based ILs ([Cnbim]Br where n = 2, 4 and 6) to calf thymus DNA was determined using fluorescence spectroscopy. It was found that the binding constant increased while the values of binding Gibbs energy decreased linearly with increase of alkyl chain length of the ILs. Based on these data, the contribution of each methylene group in the tail of ILs in IL-DNA complex was determined. Moreover, low quenching constant was observed from the competitive displacement of intercalation of ethidium bromide to DNA, hence confirming that ILs are not intercalator. The length of alkyl chain contributed to the increase in the thermal stability of DNA as observed from UV–visible spectra while the CD results indicated that DNA retained its B-form without significant change in the conformation of its base pairs. The findings show that although the stability of DNA is mainly due to the electrostatic attraction between cationic head charge group of imidazolium cation and the DNA phosphate groups, the contribution from the length of alkyl chains also plays a significant role in IL-DNA complex.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: https://doi.org/10.1016/j.molliq.2016.09.040
Publisher: Elsevier BV
Keywords: Alkyl chain length; Binding constant; DNA; Ionic liquids; Hydrophobic interaction; Gibbs energy
Depositing User: Nida Hidayati Ghazali
Date Deposited: 29 Nov 2017 09:42
Last Modified: 29 Nov 2017 09:42
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.molliq.2016.09.040
URI: http://psasir.upm.edu.my/id/eprint/55199
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