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Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense


Citation

Govender, Nisha Thopla and Mahmood, Maziah and Abu Seman, Idris and Wong, Mui Yun (2016) Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense. Polish Journal of Microbiology, 65 (3). pp. 383-388. ISSN 1733-1331

Abstract / Synopsis

Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 x 104 basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from Trichoderma harzianum (10 mg/ml) suspended in osmotic stabilizer (0.6 M potassium chloride and 20 mM dipotassium phosphate buffer) yielded the highest number of viable protoplasts (8.9 x 106 single colonies) among all possible combinations tested (regeneration media, age of mycelium, osmotic stabilizer, digestive enzyme and incubation period).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.5604/17331331.1215619
Publisher: Polish Society of Microbiologists
Keywords: Basidiocarp; Basidiospore; Incubation; Lytic enzyme
Depositing User: Nida Hidayati Ghazali
Date Deposited: 19 Dec 2017 09:56
Last Modified: 19 Dec 2017 09:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5604/17331331.1215619
URI: http://psasir.upm.edu.my/id/eprint/55190
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