UPM Institutional Repository

Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10


Citation

Md Sidek, Nurul Lyana and Joo, Shun Tan and Abbasiliasi, Sahar and Faizal Wong, Fadzlie Wong and Mustafa, Shuhaimi and Ariff, Arbakariya (2016) Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 1027. pp. 81-87. ISSN 1570-0232; ESSN: 1873-376X

Abstract

An aqueous two-phase flotation (ATPF) system based on polyethylene glycol (PEG) and sodium citrate (NaNO3C6H5O7·2H2O) was considered for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10. The effects of ATPF parameters namely phase composition, tie-line length (TLL), volume ratio between the two phases (VR), amount of crude load (CL), pH, nitrogen gas flow rate (FR) and flotation time (FT) on the performance of recovery were evaluated. BLIS was mainly concentrated into the upper PEG-rich phase in all systems tested so far. The optimum conditions for BLIS purification, which composed of PEG 8000/sodium citrate, were: TLL of 42.6, VR of 0.4, CL of 22% (w/w), pH 7, average FT of 30min and FR of 20mL/min. BLIS was partially purified up to 5.9-fold with a separation efficiency of 99% under this optimal conditions. A maximum yield of BLIS activity of about 70.3% was recovered in the PEG phase. The BLIS from the top phase was successfully recovered with a single band in SDS-gel with molecular weight of about 10-15kDa. ATPF was found to be an effective technique for the recovery of BLIS from the fermentation broth of P. acidilactici Kp10.


Download File

[img]
Preview
PDF (Abstract)
Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10.pdf

Download (6kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Halal Products Research Institute
DOI Number: https://doi.org/10.1016/j.jchromb.2016.05.024
Publisher: Elsevier BV
Keywords: Aqueous two-phase flotation; Bacteriocin-like inhibitory substance; Pediococcus acidilactici Kp10; Polyethylene glycol; Purification
Depositing User: Nida Hidayati Ghazali
Date Deposited: 26 Dec 2017 09:50
Last Modified: 26 Dec 2017 09:50
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jchromb.2016.05.024
URI: http://psasir.upm.edu.my/id/eprint/55111
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item