Simple Search:

Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats


Citation

Abdul Aziz, Siti Hajar and John, Cini Mathew and Mohamed Yusof, Nur Intan Saidaah and Nordin, Massita and Ramasamy, Rajesh and Adam, Aishah and Mohd Fauzi, Fazlin (2016) Animal model of gestational diabetes mellitus with pathophysiological resemblance to the human condition induced by multiple factors (nutritional, pharmacological, and stress) in rats. BioMed Research International, 2016 (9704607). pp. 1-14. ISSN 2314-6133; ESSN: 2314-6141

Abstract / Synopsis

This study attempts to develop an experimental gestational diabetes mellitus (GDM) animal model in female Sprague-Dawley rats. Rats were fed with high fat sucrose diet, impregnated, and induced with Streptozotocin and Nicotinamide on gestational day 0 (D0). Sleeping patterns of the rats were also manipulated to induce stress, a lifestyle factor that contributes to GDM. Rats were tested for glycemic parameters (glucose, C-peptide, and insulin), lipid profiles (total cholesterol, triglycerides, HDL, and LDL), genes affecting insulin signaling (IRS-2, AKT-1, and PCK-1), glucose transporters (GLUT-2 and GLUT-4), proinflammatory cytokines (IL-6, TNF-α), and antioxidants (SOD, CAT, and GPX) on D6 and D21. GDM rats showed possible insulin resistance as evidenced by high expression of proinflammatory cytokines, PCK-1 and CRP. Furthermore, low levels of IRS-2 and AKT-1 genes and downregulation of GLUT-4 from the initial to final phases indicate possible defect of insulin signaling. GDM rats also showed an impairment of antioxidant status and a hyperlipidemic state. Additionally, GDM rats exhibited significantly higher body weight and blood glucose and lower plasma insulin level and C-peptide than control. Based on the findings outlined, the current GDM animal model closely replicates the disease state in human and can serve as a reference for future investigations.


Download File

[img] PDF
Animal Model of Gestational Diabetes Mellitus.pdf
Restricted to Repository staff only

Download (2MB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1155/2016/9704607
Publisher: Hindawi Publishing Corporation
Keywords: Gestational diabetes mellitus (GDM); Rats; Human condition induced
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 13 Jul 2018 16:57
Last Modified: 13 Jul 2018 16:57
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2016/9704607
URI: http://psasir.upm.edu.my/id/eprint/55066
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item