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Induced pluripotent stem cells from human hair follicle keratinocytes as a potential source for in vitro hair follicle cloning


Lim, Sheng Jye and Ho, Shu Cheow and Mok, Pooi Ling and Tan, Kian Lee and Ong, Alan Han Kiat and Gan, Seng Chiew (2016) Induced pluripotent stem cells from human hair follicle keratinocytes as a potential source for in vitro hair follicle cloning. PeerJ, 4. pp. 1-17. ISSN 2167-8359


Background: Human hair follicles are important for the renewal of new hairs and their development. The generation of induced pluripotent stem cells (iPSCs) from hair follicles is easy due to its accessibility and availability. The pluripotent cells derived from hair follicles not only have a higher tendency to re-differentiate into hair follicles, but are also more suited for growth in hair scalp tissue microenvironment. Methods: In this study, human hair follicular keratinocytes were used to generate iPSCs, which were then further differentiated in vitro into keratinocytes. The derived iPSCs were characterised by using immunofluorescence staining, flow cytometry, and reverse-transcription PCR to check for its pluripotency markers expression. Results: The iPSC clones expressed pluripotency markers such as TRA-1-60, TRA-1-81, SSEA4, OCT4, SOX2, NANOG, LEFTY, and GABRB. The well-formed three germ layers were observed during differentiation using iPSCs derived from hair follicles. The successful formation of keratioctyes from iPSCs was confirmed by the expression of cytokeratin 14 marker.Discussion: Hair follicles represent a valuable keratinocytes source for in vitro hair cloning for use in treating hair balding or grafting in burn patients. Our significant findings in this report proved that hair follicles could be used to produce pluripotent stem cells and suggested that the genetic and micro-environmental elements of hair follicles might trigger higher and more efficient hair follicles re-differentiation.

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Additional Metadata

Item Type: Article
Subject: Induced pluripotent stem cell; Keratinocytes; HHFK; Re-differentiation
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.7717/peerj.2695
Publisher: PeerJ
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 28 Mar 2018 06:54
Last Modified: 28 Mar 2018 06:54
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.7717/peerj.2695
URI: http://psasir.upm.edu.my/id/eprint/54552
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