UPM Institutional Repository

In vitro study of modulatory effects of Strobilanthes crispus extracts on human cDNA-expressed cytochrome P450 2A6 (CYP2A6) and CYP3A4


pan, Yan and Hsu, Chia Jie and Koh, Rhun Yian and Ong, Chin Eng and Chieng, Jin Yu (2016) In vitro study of modulatory effects of Strobilanthes crispus extracts on human cDNA-expressed cytochrome P450 2A6 (CYP2A6) and CYP3A4. The All Results Journals: Biol, 7 (1). pp. 1-12. ISSN 2172-4784


Aim: Cytochrome P450 (CYP) 2A6 and CYP3A4 play important roles in biotransformation of endogenous substances as well as xenobiotics. Strobilanthes crispus (L.) Blume (S. crispus) has been found to have anti-cancer activities and this was suggested to be due to inhibition of enzymes involved in metabolic activation of procarcinogens. The purpose of this study was to look into the potential inhibitory effects of various extracts (aqueous, hexane, chloroform, ethyl acetate, and methanol) of S. crispus from leaf and stem on human cDNA-expressed CYP2A6 and CYP3A4 activities. Methods: The activity of CYP2A6 was examined via a fluorescence-based 7-hydroxylase coumarin assay. Meanwhile, high performance liquid chromatography (HPLC)-based testosterone 6β-hydroxylase assay was established to assess CYP3A4 activity. Results: It was shown that none of the extracts from both leaf and stem potently inhibited CYP2A6 and CYP3A4 activities with IC50values above 100μg/ml. Conclusion: The anticancer potency of S. crispus is unlikely due to the modulation of CYP2A6 and CYP3A4 activities, while other mechanisms might be involved and merits further investigation. On the other hand, potential drug-herb interactions occurring between CYP2A6 and CYP3A4 substrates and S. crispus preparations is relatively low, which requires further investigations via in vivo animal as well as clinical studies.

Download File

In vitro study of modulatory effects of extracts of Strobilanthes Crispus .pdf

Download (74kB) | Preview

Additional Metadata

Item Type: Article
Subject: CYP2A6; CYP3A4; Drug-herb interaction; Procarcinogen-activation; Strobilanthes crispus
Divisions: Faculty of Medicine and Health Science
Publisher: Society for the Improvement of Science
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 27 Mar 2018 08:10
Last Modified: 27 Mar 2018 08:10
URI: http://psasir.upm.edu.my/id/eprint/54542
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item