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In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources


Ismail, Haliza and Muniandi, Sures Kumar and Mohd Yusoff, Aziah and Hassan, Nor Hasnida and Ab Shukor, Nor Aini (2016) In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources. Dendrobiology, 75. pp. 157-165. ISSN 1641-1307


The effects of 6- Benzylaminopurine (BA), different basal medium, sucrose concentration and gelling agent were investigated for shoot induction and multiplication of Acacia auriculiformis. Nodal ex¬plants derived from 5-month-old seedlings yielded the highest shoot multiplication rate in Murashige and Skoog medium (MS) with 0.44 μM BA, 30 g/L sucrose and 2 g/L Gelrite. The highest mean number of shoots (10) and mean length of shoots (5.07mm) were also obtained in this medium. Qualitative obser¬vation of the shoots cultured in 0.44 μM BA were greener and vigorous in growth as compared to shoots cultured on higher concentrations of BA (22.2 μM). MS medium produced a significantly higher number of shoots (18) compared to Woody Plant Medium (WPM) (11) and B5 medium (10). Media solidified with different gelling agents also produced a significantly different number of shoots with 2 g/L Gelrite produced the highest number of shoots (23). The highest percentage of shoots rooted was found in the MS medium without any growth regulators (40.0%) followed by medium supplemented with Indole-3-bu¬tyric acid (IBA) at 9.84 μM and the combination of 9.84 μM IBA with 5.37 μM α-naphthalene acetic acid (NAA) (33.3%). MS medium without any plant growth regulators produced the highest mean root length (84.33mm), whereas medium supplemented with 9.84 μM IBA produced the highest mean number of roots per shoot (4.33). Out planting of in vitro rooted shoots in shredded coconut husk as the substrate gave the highest percentage of survival (90%) during acclimatization in the greenhouse.

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Additional Metadata

Item Type: Article
Subject: Basal medium; 6-Benzylaminopurine; Sucrose; Gelling agent; Micropropagation; Acacia auriculiformis
Divisions: Faculty of Forestry
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.12657/denbio.075.015
Publisher: Polish Academy of Sciences, Institute of Dendrology
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 27 Mar 2018 07:14
Last Modified: 27 Mar 2018 07:14
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.12657/denbio.075.015
URI: http://psasir.upm.edu.my/id/eprint/54540
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