Citation
Abstract
The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant difference in enzyme-linked immunosorbent assay antibody levels (p < 0.05) elicited by either pIRES/F, pIRES/F+ pIRES/HN or pIRES-F/HN at one week after the booster in specific pathogen free chickens when compared with the inactivated vaccine alone. Taken together, these results indicated that recombinant pDNA could be used to increase the efficacy of the inactivated vaccine immunization procedure.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Veterinary Medicine Institute of Bioscience |
| DOI Number: | https://doi.org/10.4142/jvs.2016.17.1.21 |
| Publisher: | The Korean Society of Veterinary Science |
| Keywords: | DNA vaccine; Newcastle disease virus; Antibody response; Inactivated vaccine |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 23 Mar 2018 04:16 |
| Last Modified: | 28 May 2020 04:23 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4142/jvs.2016.17.1.21 |
| URI: | http://psasir.upm.edu.my/id/eprint/54518 |
| Statistic Details: | View Download Statistic |
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