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Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol


Abd Manan, Fariza Aina and Abdullah, Jaafar and Nazri, Nur Nadziera and Abd Malik, Izyan Nadira and Yusof, Nor Azah and Ahmad, Ishak (2016) Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol. Malaysian Journal of Analytical Sciences, 20 (5). pp. 978-985. ISSN 1394-2506


Nanocrystalline cellulose (NCC)/chitosan composite for immobilization of tyrosinase enzyme for the determination of phenol have been developed. The NCC/Chitosan composite film on screen printed carbon electrode (SPCE) was prepared by using drop casting technique. Characterization of the modified SPCE surface was investigated by using Transmission Electron Microscopy (TEM) and Fourier Transform Infrared (FTIR), respectively. Chronoamperometric (CA) technique is used to perform the electrochemical measurements. The detection of phenols by the developed system is derived on the direct electrochemical reduction of quinones produced by enzymatic reaction. The results demonstrated that the maximum response was observed at ratio of NCC/chitosan of 1 to 1 (v/v), tyrosinase concentration of 10 mg/mL and pH buffer of 7, respectively. It was found that the developed system gave linear response in the phenol concentration range of 0.39 – 7.74 µM (slope = 28.316, R2 = 0.9808) with the detection limit of 0.38 µM. The reproducibility of the system was also estimated and the Relative Standard Deviation (RSD) was found to be at 4.27%.

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Additional Metadata

Item Type: Article
Subject: Nanocrystalline cellulose; Nanocomposite; Immobilization; Eectrochemical; Phenol
Divisions: Faculty of Science
Institute of Advanced Technology
DOI Number: https://doi.org/10.17576/mjas-2016-2005-02
Publisher: Malaysian Analytical Sciences Society
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 21 Mar 2018 03:22
Last Modified: 21 Mar 2018 03:22
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.17576/mjas-2016-2005-02
URI: http://psasir.upm.edu.my/id/eprint/54496
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