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Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC


Oon, Seok Fang and Nallappan, Meenakshii and Kassim, Nur Kartinee and Shohaimi, Shamarina and Sa'ariwijaya, Mohd Shazrul Fazry and Tee, Thiam Tsui and Cheah, Yew Hoong (2016) Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC. Biochemical and Biophysical Research Communications, 478 (3). pp. 1403-1408. ISSN 0006-291X; ESSN: 1090-2104


Hyperlipidemia is defined as the presence of either hypertriglyceridemia or hypercholesterolemia, which could cause atherosclerosis. Although hyperlipidemia can be treated by hypolipidemic drugs, they are limited due to lack of effectiveness and safety. Previous studies demonstrated that xanthorrhizol (XNT) isolated from Curcuma xanthorrhizza Roxb. reduced the levels of free fatty acid and triglyceride in vivo. However, its ability to inhibit cholesterol uptake in HT29 colon cells and adipogenesis in 3T3-L1 cells are yet to be reported. In this study, XNT purified from centrifugal TLC demonstrated 98.3% purity, indicating it could be an alternative purification method. The IC50 values of XNT were 30.81 ± 0.78 μg/mL in HT29 cells and 35.07 ± 0.24 μg/mL in 3T3-L1 adipocytes, respectively. Cholesterol uptake inhibition study using HT29 colon cells showed that XNT (15 μg/mL) significantly inhibited the fluorescent cholesterol analogue NBD uptake by up to 27 ± 3.1% relative to control. On the other hand, higher concentration of XNT (50 μg/mL) significantly suppressed the growth of 3T3-L1 adipocytes (5.9 ± 0.58%) compared to 3T3-L1 preadipocytes (81.31 ± 0.55%). XNT was found to impede adipogenesis of 3T3-L1 adipocytes in a dose-dependent manner from 3.125 to 12.5 μg/mL, where 12.5 μg/mL significantly suppressed 36.13 ± 2.1% of lipid accumulation. We postulate that inhibition of cholesterol uptake, adipogenesis, preadipocyte and adipocyte number may be utilized as treatment modalities to reduce the prevalence of lipidemia. To conclude, XNT could be a potential hypolipidemic agent to improve cardiovascular health in the future.

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Additional Metadata

Item Type: Article
Subject: Xanthorrhizol; Centrifugal TLC; HT29 colon cells; 3T3-L1 adipocytes; Cholesterol; Adipogenesis
Divisions: Faculty of Science
DOI Number: https://doi.org/10.1016/j.bbrc.2016.08.136
Publisher: Elsevier
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 20 Mar 2018 01:10
Last Modified: 20 Mar 2018 01:10
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.bbrc.2016.08.136
URI: http://psasir.upm.edu.my/id/eprint/54474
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