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Expression and characterization of thermostable glycogen branching enzyme from Geobacillus mahadia Geo-05


Citation

Mohtar, Nur Syazwani and Abdul Rahman, Mohd Basyaruddin and Raja Abd Rahman, Raja Noor Zaliha and Leow, Thean Chor and Salleh, Abu Bakar and Mat Isa, Mohd Noor (2016) Expression and characterization of thermostable glycogen branching enzyme from Geobacillus mahadia Geo-05. PeerJ, 4. pp. 1-12. ISSN 2167-8359

Abstract

The glycogen branching enzyme (EC 2.4.1.18), which catalyses the formation of α-1,6-glycosidic branch points in glycogen structure, is often used to enhance the nutritional value and quality of food and beverages. In order to be applicable in industries, enzymes that are stable and active at high temperature are much desired. Using genome mining, the nucleotide sequence of the branching enzyme gene (glgB) was extracted from the Geobacillus mahadia Geo-05 genome sequence provided by the Malaysia Genome Institute. The size of the gene is 2013 bp, and the theoretical molecular weight of the protein is 78.43 kDa. The gene sequence was then used to predict the thermostability, function and the three dimensional structure of the enzyme. The gene was cloned and overexpressed in E. coli to verify the predicted result experimentally. The purified enzyme was used to study the effect of temperature and pH on enzyme activity and stability, and the inhibitory effect by metal ion on enzyme activity. This thermostable glycogen branching enzyme was found to be most active at 55 °C, and the half-life at 60 °C and 70 °C was 24 h and 5 h, respectively. From this research, a thermostable glycogen branching enzyme was successfully isolated from Geobacillus mahadia Geo-05 by genome mining together with molecular biology technique.


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Additional Metadata

Item Type: Article
Subject: 1-4-alpha-glucan branching enzyme; His-patch thioredoxin; Geobacillus sp; Glycogen branching enzyme; Genome mining
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: https://doi.org/10.7717/peerj.2714
Publisher: PeerJ
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 08 Mar 2018 04:32
Last Modified: 08 Mar 2018 04:32
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.7717/peerj.2714
URI: http://psasir.upm.edu.my/id/eprint/54244
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