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Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain


Leong, Jia Wen and Abdullah, Syahril and Ling, King Hwa and Cheah, Pike See (2016) Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain. Neural Plasticity, 2016 (1951250). pp. 1-16. ISSN 2090-5904; ESSN: 1687-5443


MicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. In situ hybridization analysis showed that miR-344b and miR-344c were expressed in the germinal layer during embryonic brain developmental stages. In contrast, miR-344b was not detectable in the adult brain while miR-344c was expressed exclusively in the adult olfactory bulb and cerebellar granular layer. Stem-loop RT-qPCR analysis of whole brain RNAs showed that expression of the miR-344b and miR-344c was increased as brain developed throughout the embryonic stage and maintained at adulthood. Further investigation showed that these miRNAs were expressed in adult organs, where miR-344b and miR-344c were highly expressed in pancreas and brain, respectively. Bioinformatics analysis suggested miR-344b and miR-344c targeted Olig2 and Otx2 mRNAs, respectively. However, luciferase experiments demonstrated that these miRNAs did not target Olig2 and Otx2 mRNAs. Further investigation on the locality of miR-344b and miR-344c showed that both miRNAs were localized in nuclei of immature neurons. In conclusion, miR-344b and miR-344c were expressed spatiotemporally during mouse brain developmental stages.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1155/2016/1951250
Publisher: Hindawi Publishing Corporation
Keywords: Spatiotemporal expression; Molecular characterization; miR-344b; miR-344c; Developing mouse brain; Mouse brain
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 15 Feb 2018 03:59
Last Modified: 15 Feb 2018 03:59
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2016/1951250
URI: http://psasir.upm.edu.my/id/eprint/53863
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