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Seroprevalence and molecular detection of leptospirosis from a dog shelter


Khor, Kuan Hua and Tan, Wei Xian and Lau, Seng Fong and Roslan, Mohd Azri and Radzi, Rozanaliza and Bejo, Siti Khairani and Bahaman, Abdul Rani (2016) Seroprevalence and molecular detection of leptospirosis from a dog shelter. Tropical Biomedicine, 33 (2). pp. 276-284. ISSN 0127-5720


A study on seroprevalence and molecular detection of canine leptospirosis was carried out in a dog population (randomly selected n=80 dogs) from an animal shelter X. All the dogs in Shelter X appeared clinically healthy. Eighty blood samples were obtained and their serum were serologically examined using Microscopic Agglutination Test (MAT) against 10 Leptospira serovars. Plasma samples obtained were subjected to Polymerase Chain Reaction (PCR) assay. Three out of 80 dogs (3.8%) tested positive for L. bataviae based on MAT at a titer of 1:80. The seroprevalence of 9 other Leptospira serovars was not evident in this study. All the dogs tested negative against leptospirosis with PCR assay. In conclusion, canine leptospirosis was detected in dogs in this animal shelter. L. bataviae was identified as the infecting serovar. To our knowledge, this is the second report of serovar Bataviae infection in dogs in Malaysia. The 3 dogs in our study could possibly be a source of leptospiral infection to other dogs and may shed the bacteria into the environment. This serovar is not available in canine vaccination programs, therefore the dogs are not protected from this disease. Further investigation is warranted to determine whether the infected dogs are carriers of this serovar.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Malaysian Society of Parasitology and Tropical Medicine
Keywords: Seroprevalence; Molecular detection; Leptospirosis; Dog shelter
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 02 Feb 2018 03:40
Last Modified: 02 Feb 2018 03:40
URI: http://psasir.upm.edu.my/id/eprint/53797
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