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Sensitive detection of multiple pathogens using a single DNA probe


Nordin, Noordiana and Yusof, Nor Azah and Abdullah, Jaafar and Radu, Son and Hushiarian, Roozbeh (2016) Sensitive detection of multiple pathogens using a single DNA probe. Biosensors and Bioelectronics, 86 (1). pp. 398-405. ISSN 0956-5663; ESSN: 1873-4235


A simple but promising electrochemical DNA nanosensor was designed, constructed and applied to differentiate a few food-borne pathogens. The DNA probe was initially designed to have a complementary region in Vibrio parahaemolyticus (VP) genome and to make different hybridization patterns with other selected pathogens. The sensor was based on a screen printed carbon electrode (SPCE) modified with polylactide-stabilized gold nanoparticles (PLA-AuNPs) and methylene blue (MB) was employed as the redox indicator binding better to single-stranded DNA. The immobilization and hybridization events were assessed using differential pulse voltammetry (DPV). The fabricated biosensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0×10−9–2.0×10−13 M with a detection limit of 5.3×10−12 M. The relative standard deviation for 6 replications of DPV measurement of 0.2 µM complementary DNA was 4.88%. The fabricated DNA biosensor was considered stable and portable as indicated by a recovery of more than 80% after a storage period of 6 months at 4–45 °C. Cross-reactivity studies against various food-borne pathogens showed a reliably sensitive detection of VP.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Science
Institute of Advanced Technology
DOI Number: https://doi.org/10.1016/j.bios.2016.06.077
Publisher: Elsevier
Keywords: Electrochemical DNA biosensor; Polylactide-stabilized gold nanoparticles (PLA-AuNPs); Nucleic acid hybridization detection; Methylene blue; Food-borne pathogens
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 01 Feb 2018 10:23
Last Modified: 01 Feb 2018 10:23
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.bios.2016.06.077
URI: http://psasir.upm.edu.my/id/eprint/53794
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