Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system

Citation

Lin, Yu Kiat and Show, Pau Loke and Yap, Yee Jiun and Ariff, Arbakariya and Mohammad Annuar, Mohammad Suffian and Lai, Oi Ming and Tang, Teck Kim and Juan, Joon Ching and Ling, Tau Chuan (2016) Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system. Journal of Bioscience and Bioengineering, 121 (6). pp. 692-696. ISSN 1389-1723; ESSN: 1347-4421

Abstract

Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT = 81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase = 0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Biotechnology and Biomolecular Sciences
DOI Number:	https://doi.org/10.1016/j.jbiosc.2015.11.001
Publisher:	Elsevier
Keywords:	Aqueous two-phase system; Bacillus cereus; Cyclodextrin; Cyclodextrin glycosyltransferease; Extractive bioconversion
Depositing User:	Nurul Ainie Mokhtar
Date Deposited:	04 Jan 2018 06:04
Last Modified:	04 Jan 2018 06:04
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jbiosc.2015.11.001
URI:	http://psasir.upm.edu.my/id/eprint/53680
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