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Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc


Citation

Bande, Faruku and Arshad, Siti Suri and Bejo, Mohd Hair and Kadkhodaei, Saeid and Omar, Abdul Rahman (2016) Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc. Advances in Bioinformatics, 2016. art. no. 5484972. pp. 1-5. ISSN 1687-8027; ESSN: 1687-8035

Abstract / Synopsis

Bioinformatic analysis was used to predict antigenic B-cell and T-cell epitopes within the S1 glycoprotein of M41 and CR88 IBV strains. A conserved linear B-cell epitope peptide, , was identified in M41 IBV strains while three such epitopes types namely, , , and , were predicted in CR88 IBV strains. Analysis of MHCI binding peptides in M41 IBV strains revealed the presence of 15 antigenic peptides out of which 12 were highly conserved in 96–100% of the total M41 strains analysed. Interestingly three of these peptides, GGPITYKVM208, WFNSLSVSI356, and YLADAGLAI472, relatively had high antigenicity index (>1.0). On the other hand, 11 MHCI binding epitope peptides were identified in CR88 IBV strains. Of these, five peptides were found to be highly conserved with a range between 90% and 97%. However, WFNSLSVSL358, SYNISAASV88, and YNISAASVA89 peptides comparably showed high antigenicity scores (>1.0). Combination of antigenic B-cells and T-cells peptides that are conserved across many strains as approach to evoke humoral and CTL immune response will potentially lead to a broad-based vaccine that could reduce the challenges in using live attenuated vaccine technology in the control of IBV infection in poultry.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.1155/2016/5484972
Publisher: Hindawi Publishing Corporation
Keywords: Novel B-cells; T-cells; S1-spike glycoprotein; M41; CR88; Bronchitis virus; Peptide vacc
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 20 Nov 2017 11:57
Last Modified: 20 Nov 2017 11:57
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2016/5484972
URI: http://psasir.upm.edu.my/id/eprint/53633
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