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Optimization and maximization of hexavalent molybdenum reduction to Mo-blue by Serratia sp. strain MIE2 using response surface methodology


Citation

Halmi, Mohd Izuan Effendi and Sheikh Abdullah, Siti Rozaimah and Wasoh, Helmi and Wan Johari, Wan Lutfi and Mohamad Ali, Mohd Shukuri and Shaharuddin, Noor Azmi and Shukor, Mohd Yunus (2016) Optimization and maximization of hexavalent molybdenum reduction to Mo-blue by Serratia sp. strain MIE2 using response surface methodology. Rendiconti Lincei. Scienze Fisiche e Naturali, 27 (4). pp. 697-709. ISSN 2037-4631; ESSN: 1720-0776

Abstract

Molybdenum has long been known to be toxic to ruminants, but not to humans. However, more recently it has been increasingly reported that molybdenum shows toxic effects to reproductive organs of fish, mouse and even humans. Hence, its removal from the environment is highly sought after. In this study, response surface methodology (RSM) was successfully applied in the optimization and maximization of Mo6+ reduction to Mo-blue by Serratia sp. MIE2 for future bioremediation application. The optimal conditions predicted by RSM were 20 mM molybdate, 3.95 mM phosphate, pH 6.25 and 25 g l−1 sucrose with absorbance of 19.53 for Mo-blue production measured at 865 nm. The validation experimental run of the predicted optimal conditions showed that the maximum Mo-blue production occurred at absorbance of 20.87, with a 6.75 % deviation from the predicted value obtained from RSM. Molybdate reduction was successfully maximized using RSM with molybdate reduction before and after optimization using RSM showing Mo-blue production starting at the absorbance value of 10.0 at 865 nm going up to an absorbance value above 20.87. The modelling kinetics of Mo6+ reduction showed that Teissier was the best model, with calculated Pmax, Ks and Ki values of 1.97 Mo-blue per hour, 5.79 mM and 31.48 mM, respectively.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Environmental Studies
DOI Number: https://doi.org/10.1007/s12210-016-0552-4
Publisher: Springer - Verlag Italia Srl
Keywords: Bioremediation; Response surface methodology; Hexavalent molybdenum; Modelling kinetics
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 31 Oct 2017 04:06
Last Modified: 31 Oct 2017 04:06
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s12210-016-0552-4
URI: http://psasir.upm.edu.my/id/eprint/53444
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