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Optimisation of supercritical fluid extraction of astaxanthin from Penaeus monodon waste using ethanol-modified carbon dioxide


Citation

Radzali, Shazana Azfar and Markom, Masturah and Baharin, Badlishah Shah and Othman, Rashidi and Abdul Rahman, Russly (2016) Optimisation of supercritical fluid extraction of astaxanthin from Penaeus monodon waste using ethanol-modified carbon dioxide. Journal of Engineering Science and Technology, 11 (5). pp. 722-736. ISSN 1823-4690

Abstract

Some studies demonstrated that astaxanthin surpasses the antioxidant benefits of beta-carotene, zeaxanthin, canthaxanthin, vitamin C, and vitamin E. Penaeus monodon (Tiger shrimp) is one of the most valuable traded crustacean products in which astaxanthin can be found in its by-products. The extraction of thermolabile compound like carotenoids at lower temperatures through supercritical carbon dioxide (SC-CO2) can reduce the potential isomerization and degradation of the extraction product. In this study, astaxanthin had been extracted using SC-CO2 with 15% (v/v) ethanol as an entrainer and the recovered astaxanthin was analyzed using High performance liquid chromatography (HPLC). A central composite design (CCD) was employed to study the effect of three SC-CO2 parameters namely temperature (X1) from 40 to 80°C, pressure (X2) from 150 to 250 bar and extraction flow rate (X3) from 1 to 3 ml/min on the astaxanthin complex yield, (Y1) and free astaxanthin content, (Y2). The nonlinear regression equations were significantly (p<0.05) fitted for both responses with high R2 (>0.9261), which had no indication of lack of fit. The results indicated that a combined set of values of temperature (56.88°C), pressure (215.68 bar) and extraction flow rate (1.89 ml/min) was predicted to provide the optimum region in terms of astaxanthin complex yield, (58.50 ± 2.62 μg/g ) and free astaxanthin content (12.20 ± 4.16 μg/g) studied.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: School of Engineering, Taylor’s University College
Keywords: Penaeus monodon; Astaxanthin; Carotenoids; SFE; Optimization; HPLC
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 31 Oct 2017 03:31
Last Modified: 31 Oct 2017 03:31
URI: http://psasir.upm.edu.my/id/eprint/53442
Statistic Details: View Download Statistic

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