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Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent


Citation

Baradaran, Ali and Yusoff, Khatijah and Shafee, Norazizah and Abdul Rahim, Raha (2016) Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent. Journal of Cancer, 7 (4). pp. 462-466. ISSN 1837-9664

Abstract

The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) with its immunotherapeutic activities and sialic acid binding abilities is a promising cancer adjuvant. The HN was surfaced displayed on Lactococcus lactis and its cancer targeting ability was investigated via attachment to the MDA-MB231 breast cancers. To surface display the HN protein on the bacterial cell wall, HN was fused to N-acetylmuraminidase (AcmA) anchoring motif of L. lactis and expressed in Chinese hamster ovary cells. The expressed recombinant fusion proteins were purified and mixed with a culture of L. lactis and Lactobacillus plantarum. Immunofluorescence assay showed the binding of the recombinant HN-AcmA protein on the surface of the bacterial cells. The bacterial cells carrying the HN-AcmA protein interacted with the MDA-MB231 breast cancer cells. Direct and fluorescent microscopy confirmed that L. lactis and Lb. plantarum surface displaying the recombinant HN were attached to the breast cancer MDA-MB231 cells, providing evidence for the potential ability of HN in targeting to cancer cells.


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Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
DOI Number: https://doi.org/10.7150/jca.13566
Publisher: Ivyspring International Publisher
Keywords: Hemagglutinin-neuraminidase; Cancer targeting; Lactococcus lactis; Surface display
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 25 Oct 2017 08:13
Last Modified: 25 Oct 2017 08:13
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.7150/jca.13566
URI: http://psasir.upm.edu.my/id/eprint/53382
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