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Transcriptional expression of three putative pathogenesis-related proteins in leaves of rubber tree (Hevea brasiliensis) inoculated with Neofusicoccum ribis


Citation

A. I. C., Nyaka Ngobisa and Godswill, Ntsomboh Ntsefong and Wong, Mui Yun and M. Z., Dzarifah and P. A., Owona Ndongo (2016) Transcriptional expression of three putative pathogenesis-related proteins in leaves of rubber tree (Hevea brasiliensis) inoculated with Neofusicoccum ribis. Journal of Applied Biology & Biotechnology, 4 (2). pp. 11-15. ISSN 2455-7005; ESSN: 2347-212X

Abstract / Synopsis

Pathogenesis related-proteins (PR-proteins) and enzymes are important tools for understanding the molecular markers of plant response to external factors. In this study, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was optimized to detect the expressions of two PR-proteins and one enzyme extracted from the leaf RNA of two rubber clones (PB 350 and RRIM200) at four-period intervals. The expression patterns of enzymes β-1,3-glucanase (Glu), chitinase (Chit), and phenylalanine ammonia lyase (PAL) in the leaf tissues of the clones RRIM 2002 and PB 350 to Neofusicoccum ribis were expressed by up and down regulations and varied with time. The trial inoculation indicated that the gene expressions were significantly higher in tolerant clones (RRIM 2002) than in the susceptible clones (PB 350). Gene expression analysis of the biomarkers revealed that the earlier detection markers in infections occurred within 5–10 days post infection and showed significant correlation with disease development, ranging from 1.67–3% in the RRIM 2002 clone and approximately 8.33% in the PB 350 clone. This is the first study on the infection and up-regulation of gene expression in rubber leaves infected with N. ribis isolate SK10 (ICMP 20078). This study showed the importance of exploring the SK10 isolate as an indicator of infection ability and positive fungal–host interaction.


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Official URL: http://www. jabonline.in

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.7324/JABB.2016.40202
Publisher: Open Science Publishers
Keywords: Gene expression; RT-PCR; Neofusicoccum ribis; Pathogenesis-related protein
Depositing User: Nida Hidayati Ghazali
Date Deposited: 31 Oct 2017 10:10
Last Modified: 31 Oct 2017 10:10
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.7324/JABB.2016.40202
URI: http://psasir.upm.edu.my/id/eprint/53152
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