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Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars


Sahebi, Mahbod and Musa, Mohamed Hanafi and Yusop, Mohd Rafii and Azizi, Parisa and Abiri, Rambod and Kalhori, Nahid and Atabaki, Narges (2017) Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars. BioMed Research International, 2017. art. no. 9064129. pp. 1-13. ISSN 2314-6133; ESSN: 2314-6141


Silicon (Si) is one of the most prevalent elements in the soil. It is beneficial for plant growth and development, and it contributes to plant defense against different stresses. The Lsi1 gene encodes a Si transporter that was identified in a mutant Japonica rice variety. This gene was not identified in fourteen Malaysian rice varieties during screening. Then, a mutant version of Lsi1 was substituted for the native version in the three most common Malaysian rice varieties, MR219, MR220, and MR276, to evaluate the function of the transgene. Real-time PCR was used to explore the differential expression of Lsi1 in the three transgenic rice varieties. Silicon concentrations in the roots and leaves of transgenic plants were significantly higher than in wild-type plants. Transgenic varieties showed significant increases in the activities of the enzymes SOD, POD, APX, and CAT; photosynthesis; and chlorophyll content; however, the highest chlorophyll A and B levels were observed in transgenic MR276. Transgenic varieties have shown a stronger root and leaf structure, as well as hairier roots, compared to the wild-type plants. This suggests that Lsi1 plays a key role in rice, increasing the absorption and accumulation of Si, then alters antioxidant activities, and improves morphological properties.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.1155/2017/9064129
Publisher: Hindawi Publishing Corporation
Keywords: Silicon; Rice; Lsi1; Silicon transporter gene
Depositing User: Nabilah Mustapa
Date Deposited: 06 Jun 2017 08:43
Last Modified: 06 Jun 2017 08:43
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2017/9064129
URI: http://psasir.upm.edu.my/id/eprint/52422
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