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Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR


Citation

Bilung, Lesley Maurice and Radu, Son and Bahaman, Abdul Rani and Abdul Rahim, Raha and Napis, Suhaimi and Wong, Clemente Michael Vui Ling and Tanil, Gwendelynne Bulan and Nishibuchi, Mitsuaki (2005) Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR. FEMS Microbiology Letters, 252 (1). pp. 85-88. ISSN 0378-1097; ESSN: 1574-6968

Abstract / Synopsis

This study aimed to determine the occurrence of Vibrio parahaemolyticus in cockles (Anadara granosa) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin (tdh) and TDH-related hemolysin genes (trh) using PCR. Of 100 samples, 62 were positive for the presence of V. parahaemolyticus with an MPN (most probable number) value greater than 3.0 (>1100 MPN per g). The PCR analysis revealed 2 samples to be positive for the tdh gene and 11 to be positive for the trh gene. Hence, these results demonstrate the presence of pathogenic V. parahaemolyticus in cockles harvested in the study area and reveal the potential risk of illness associated with their consumption.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1016/j.femsle.2005.08.053
Publisher: Elsevier
Keywords: Cockles; PCR; Tdh; Trh; Vibrio parahaemolyticus
Depositing User: Nabilah Mustapa
Date Deposited: 05 Jun 2017 17:34
Last Modified: 05 Jun 2017 17:34
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.femsle.2005.08.053
URI: http://psasir.upm.edu.my/id/eprint/52353
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