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Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourizing Bacillus sp. strain Neni-10 in soils from West Sumatera, Indonesia


Citation

Mansur, Rusnam and Gusmanizar, Neni and Roslan, Muhamad Akhmal Hakim and Ahmad, Siti Aqlima and Abd. Shukor, Mohd Yunus (2017) Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourizing Bacillus sp. strain Neni-10 in soils from West Sumatera, Indonesia. Tropical Life Sciences Research, 28 (1). pp. 69-90. ISSN 1985-3718; ESSN: 2180-4249

Abstract

A molybdenum reducing bacterium with the novel ability to decolorise the azo dye Metanil Yellow is reported. Optimal conditions for molybdenum reduction were pH 6.3 and at 34°C. Glucose was the best electron donor. Another requirement includes a narrow phosphate concentration between 2.5 and 7.5 mM. A time profile of Mo-blue production shows a lag period of approximately 12 hours, a maximum amount of Mo-blue produced at a molybdate concentration of 20 mM, and a peak production at 52 h of incubation. The heavy metals mercury, silver, copper and chromium inhibited reduction by 91.9, 82.7, 45.5 and 17.4%, respectively. A complete decolourisation of the dye Metanil Yellow at 100 and 150 mg/L occurred at day three and day six of incubations, respectively. Higher concentrations show partial degradation, with an approximately 20% decolourisation observed at 400 mg/L. The bacterium is partially identified based on biochemical analysis as Bacillus sp. strain Neni-10. The absorption spectrum of the Mo-blue suggested the compound is a reduced phosphomolybdate. The isolation of this bacterium, which shows heavy metal reduction and dye-decolorising ability, is sought after, particularly for bioremediation.


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Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourizing Bacillus sp. strain Neni-10 in soils from West Sumatera, Indonesia.pdf

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Penerbit Universiti Sains Malaysia
Keywords: Molybdenum-reducing; Molybdenum blue; Bacillus sp.; Azo dye; Metanil Yellow
Depositing User: Nabilah Mustapa
Date Deposited: 03 May 2017 09:20
Last Modified: 03 May 2017 09:20
URI: http://psasir.upm.edu.my/id/eprint/51936
Statistic Details: View Download Statistic

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