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Study on the spectrophotometric detection of free fatty acids in palm oil utilizing enzymatic reactions


Azeman, Nur Hidayah and Yusof, Nor Azah and Abdullah, Jaafar and Yunus, Robiah and Hamidon, Mohd Nizar and Hajian, Reza (2015) Study on the spectrophotometric detection of free fatty acids in palm oil utilizing enzymatic reactions. Molecules, 20 (7). pp. 12328-12340. ISSN 1420-3049


In this paper, a comprehensive study has been made on the detection of free fatty acids (FFAs) in palm oil via an optical technique based on enzymatic aminolysis reactions. FFAs in crude palm oil (CPO) were converted into fatty hydroxamic acids (FHAs) in a biphasic lipid/aqueous medium in the presence of immobilized lipase. The colored compound formed after complexation between FHA and vanadium (V) ion solution was proportional to the FFA content in the CPO samples and was analyzed using a spectrophotometric method. In order to develop a rapid detection system, the parameters involved in the aminolysis process were studied. The utilization of immobilized lipase as catalyst during the aminolysis process offers simplicity in the product isolation and the possibility of conducting the process under extreme reaction conditions. A good agreement was found between the developed method using immobilized Thermomyces lanuginose lipase as catalyst for the aminolysis process and the Malaysian Palm Oil Board (MPOB) standard titration method (R2 = 0.9453).

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Official URL or Download Paper: http://www.mdpi.com/1420-3049/20/7/12328

Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Faculty of Science
Institute of Advanced Technology
DOI Number: https://doi.org/10.3390/molecules200712328
Publisher: MDPI
Keywords: Free fatty acid; Enzymatic reaction; Aminolysis; Palm oil; Lipase; Spectrophotometry determination
Depositing User: Nabilah Mustapa
Date Deposited: 06 Sep 2017 07:52
Last Modified: 06 Sep 2017 07:52
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules200712328
URI: http://psasir.upm.edu.my/id/eprint/51771
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