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Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling


Citation

Mada, Khumran Armiya'u and Degu, Nurhusien Yimer and Yusoff, Rosnina and Omar, Mohamed Ariff and Haron, Abd Wahid and Kaka, Asmatullah and Ebrahimi, Mahdi and Aliyu, Abdulrasheed Bello (2017) Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling. Pertanika Journal of Tropical Agricultural Science, 40 (1). pp. 131-142. ISSN 1511-3701; ESSN: 2231-8542

Abstract

The effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on chilled bull semen for 72 h at 4°C in Bioxcell® (BX), Tris egg-yolk- (TEY) and citrate egg-yolk- (CEY) based semen extenders were studied. Twenty-two ejaculates collected from three bulls, each extended using three extenders with BHT at 0 (control), 0.5, 1.0, 1.5, 2.0 and 3.0 mM/mL, were evaluated for sperm quality parameters. Extended semen was packaged into 0.25 mL straws containing 20 x 106 spermatozoa and chilled for 72 h. Four random straws each from the control and treatment groups were warmed at 37°C, pooled and evaluated using a computer-assisted semen analyser (IVOS Hamilton Thorne Biosciences) for general and progressive motilities, and for morphology, viability and acrosome damage using eosin-nigrosin stain under phase-contrast microscope. Results showed that sperm morphology, viability and protection of acrosome damage were significantly improved (p<0.05) at BHT concentrations of 0.5 in BX and 1.0 mM/mL in TEY and CEY compared to the controls. The BHT also showed a potential positive effect on progressive motility at 0.5 mM/mL in BX and 1.0 mM/mL in TEY and CEY. High concentrations of BHT (2.0 and 3.0 mM/mL), however, produced deteriorative effects on the sperm parameters in all the extenders. In conclusion, BHT when added at 0.5 mM/mL in BX and 1.0 mM/mL concentration to TEY and CEY extenders improved the quality parameters of bull chilled semen.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Universiti Putra Malaysia Press
Keywords: Antioxidant; Bull; Butylated hydroxytoluene; Extender; Oxidation; Semen
Depositing User: Nabilah Mustapa
Date Deposited: 05 Apr 2017 05:22
Last Modified: 05 Apr 2017 05:22
URI: http://psasir.upm.edu.my/id/eprint/51626
Statistic Details: View Download Statistic

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