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Kojic acid production by Aspergillus flavus using gelatinized and hydrolyzed sago starch as carbon sources


Citation

Mohamad, Rosfarizan and Ariff, Arbakariya and Hassan, Mohd Ali and Abdul Karim, Mohamed Ismail (1998) Kojic acid production by Aspergillus flavus using gelatinized and hydrolyzed sago starch as carbon sources. Folia Microbiologica, 43 (5). pp. 459-464. ISSN 0015-5632; ESSN: 1874-9356

Abstract

Direct conversion of gelatinized sago starch into kojic acid byAspergillus flavus strain having amylolytic enzymes was carried out at two different scales of submerged batch fermentation in a 250-mL shake flask and in a 50-L stirred-tank fermentor. For comparison, fermentations were also carried out using glucose and glucose hydrolyzate from enzymic hydrolysis of sago starch as carbon sources. During kojic acid fermentation of starch, starch was first hydrolyzed to glucose by the action of α-amylase and glucoamylase during active growth phase. The glucose remaining during the production phase (non-growing phase) was then converted to kojic acid. Kojic acid production (23.5g/L) using 100 g/L sago starch in a shake flask was comparable to fermentation of glucose (31.5 g/L) and glucose hydrolyzate (27.9 g/L) but in the 50-L fermentor was greatly reduced due to non-optimal aeration conditions. Kojic acid production using glucose was higher in the 50-L fermentor than in the shake flask.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Biotechnology
DOI Number: https://doi.org/10.1007/BF02820791
Publisher: Springer
Keywords: Kojic acid; Aspergillus flavus; Sago starch; Fermentation
Depositing User: Nabilah Mustapa
Date Deposited: 04 Jul 2017 03:03
Last Modified: 04 Jul 2017 03:03
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/BF02820791
URI: http://psasir.upm.edu.my/id/eprint/51214
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