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Enzymatic synthesis of fatty esters by alkylated lipase


Basri, Mahiran and Ampon, Kamaruzaman and Wan Yunus, Wan Md. Zin and Abdul Razak, Che Nyonya and Salleh, Abu Bakar (1997) Enzymatic synthesis of fatty esters by alkylated lipase. Journal of Molecular Catalysis B: Enzymatic, 3 (1-4). pp. 171-176. ISSN 1381-1177


Lipase from Candida rugosa was chemically modified by reductive alkylation with aldehydes of various chain lengths. The derivatised lipases showed a higher esterification activity compared to native enzyme. The degree of activity enhancement depended on the type and molecular weight of the modifiers used and the degree of modification of the enzyme. They exhibited higher activities in non-polar than in polar solvents. The optimum esterification temperature and preference of fatty acids as acyl donors of the derivatised lipases were very similar to those of native enzyme. Lipase derivatised with dodecyldehyde was more thermostable than those modified with acetaldehyde. Alkylated lipases are relatively more stable in organic solvents than the native enzyme.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Science and Environmental Studies
DOI Number: https://doi.org/10.1016/S1381-1177(96)00054-9
Publisher: Elsevier Science
Keywords: Reductive alkylation; Esterification; Selectivity; Stability
Depositing User: Nabilah Mustapa
Date Deposited: 27 Apr 2017 09:53
Last Modified: 27 Apr 2017 09:53
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/S1381-1177(96)00054-9
URI: http://psasir.upm.edu.my/id/eprint/51109
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