UPM Institutional Repository

Purification and properties of pectinesterase from soursop (Anona muricata) pulp


Misman, Siti Arbaisah and Ali, Asbi and Abdul Hamid, Junainah and Bakar, Jamilah (1997) Purification and properties of pectinesterase from soursop (Anona muricata) pulp. Food Chemistry, 59 (1). pp. 33-40. ISSN 0308-8146


Two forms of pectinesterase were purified using the techniques of ammonium sulphate fractionation, ion-exchange chromatography and gel filtration. PE I had a specific activity of approximately 4 units mg−1 (43-fold), that of PE II was 6.4 units mg−1 (229-fold). These pectinesterases (PE I and PE II) had approximate molecular weights of 29 100 and 24 100, respectively, as estimated by gel filtration, and 31 000 and 28 000, respectively, as estimated by sodium dodecyl sulphate polyacrylamide electrophoresis. The optimum temperature for enzyme activity was shown to be 60 °C for both PE I and PE II. The activation energies of PE I and PE II were calculated as 36 kJ mol−1 and 42 kJ mol−1, respectively. The optimum pH values for both pectinesterases lie within the range 7.0–8.0. The Km value for PE I was 0.52 mg ml−1 and 0.0843 mg ml−1 for PE II. PE I had a maximum velocity (Vmax) of 154 μmol mg−1 min−1, and PE II a Vmax of 726 μmol mg−1 min−1.

Download File

PDF (Abstract)

Download (48kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Biotechnology
DOI Number: https://doi.org/10.1016/S0308-8146(96)00043-X
Publisher: Elsevier Science
Keywords: Pectinesterase; Purification; Anona muricata
Depositing User: Nabilah Mustapa
Date Deposited: 27 Apr 2017 10:08
Last Modified: 27 Apr 2017 10:08
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/S0308-8146(96)00043-X
URI: http://psasir.upm.edu.my/id/eprint/51107
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item