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Separation of cyclodextrins (CDs) using aqueous two-phase system


Ng, Hui Suan and Mokhtar, Mohd Noriznan and Ariff, Arbakariya and Ling, Tau Chuan (2012) Separation of cyclodextrins (CDs) using aqueous two-phase system. In: International Conference on Agricultural and Food Engineering for Life (Cafei2012), 26-28 Nov. 2012, Palm Garden Hotel, Putrajaya. (pp. 319-322).


Separation of three different types of cyclodextrins (CDs) [α-,β-, and γ- CD] were investigated in the polyethylene glycol (PEG) 20000/ dextran T500 aqueous two-phase system (ATPS) with an aim to study the partitioning behavior of CDs in the absence of the CDs producing enzyme, cyclodextrin glycosyltransferase (CGTase). The ATPS developed (7.7% (w/w) PEG 20000, 10.3% Dextran T500, with volume ratio, VR of 4.0 was the optimum ATPS for the sago starch bioconversion of Bacillus cereus CGTase. This preliminary study was useful for the further kinetic studies of the sago starch conversion into CDs by the CGTase in an ATPS. The study showed that all types of CDs exhibit the PEG-rich phase preference with the high partition coefficient (KCD) over time. However, difference in the partition coefficients between these 3 CDs was observed, suggesting that their partitioning behaviour in the ATPS were different as a result of the difference in the hydrophobic interactions with the phase components and solubility of CDs. The exclusive partition of CDs into the PEG-rich phase is beneficial for the extractive bioconversion of starch in ATPS.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Publisher: Faculty of Engineering, Universiti Putra Malaysia
Keywords: Separation; Cyclodextrin; Bacillus cereus; Cyclodextrin glycosyltransferase; Aqueous two-phase
Depositing User: Nabilah Mustapa
Date Deposited: 02 Mar 2017 06:18
Last Modified: 02 Mar 2017 06:18
URI: http://psasir.upm.edu.my/id/eprint/50669
Statistic Details: View Download Statistic

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