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Zerumbone induces apoptosis in human acute leukemia Jurkat T-cell line


Rahman, Heshu Sulaiman and Abdullah, Rasedee and Abdul, Ahmad Bustamam and Othman, Hemn Hassan and Allaudin, Zeenathul Nazariah and Wun, H. C. and Yeap, Swee Keong (2012) Zerumbone induces apoptosis in human acute leukemia Jurkat T-cell line. In: International Conference on One Health and 24th VAM Congress 2012, 21-23 Sept. 2012, Marriott Putrajaya. (pp. 301-302).


Zerumbone (ZER) is a potential anticancer natural phytochemical compound, isolated from Zingiber zerumbet (L.) Smith. In this investigation, the anticancer properties of ZER were evaluated on cancer cells of T-acute lymphoblastic leukemia, Jurkat cells using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and microscopic investigation (Flouresent microscope and scanning electron microscope). The results showed that ZER has cytotoxic effect against Jurkat cell in time dependent manner (24, 48 and 72h) with an IC50 of 11.875±0.178μg/ml, 8.593±0.480μg/ml and 5.397±0.431μg/ml respectively. Comparatively, doxorubicin (positive control) imposed an inhibitory effect on Jurkat cells with an IC50 of 1.514±0.070μg/ml after 72hr incubation. The results also revealed that ZER induced apoptosis time dependently in Jurkat cells using Annexin-v double staining assay. The current finding conducted that ZER with its unique chemical structure and versatile pharmacological activities might be helpful for improving the usefulness of anticancer agents in the therapy of leukemia.

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Additional Metadata

Item Type: Conference or Workshop Item (Poster)
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Keywords: Zerumbone; MTT assay; Annexin-V test; Microscopic investigation; Leukemia
Depositing User: Nabilah Mustapa
Date Deposited: 02 Feb 2017 05:00
Last Modified: 02 Feb 2017 05:00
URI: http://psasir.upm.edu.my/id/eprint/50209
Statistic Details: View Download Statistic

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