UPM Institutional Repository

DNA fingerprinting of red jungle fowl, village chicken and broilers


Mohd Lila, Mohd Azmi and Ali, A. S. and Kheng, W. K. (2000) DNA fingerprinting of red jungle fowl, village chicken and broilers. Asian-Australasian Journal of Animal Sciences, 13 (8). pp. 1040-1043. ISSN 1011-2367; ESSN: 1976-5517


The genomic mapping of Red Jungle Fowl (Gallus gallus), local Village Chicken, and broiler was carried out by random amplified polymorphism DNA (RAPD) technique. Two different sets of arbitrary primers were used (Operon OPAO1-20 and Genemed GM01-50). All the genomes of the three species of chickens were amplified with OPA01-20 primers. The genomes of the Red Jungle Fowl and local Village Chicken were further amplified with GM01-50 primers. Analysis of the results based on band sharing (BS) and the molecular size of individually amplified DNA fragments showed that Red Jungle Fowl and local Village Chicken shared the species similarity of 66% with Operon primers 01-20, 64% between local Village Chicken and broiler, and 63% when DNA bands between Red Jungle Fowl and broiler were compared. With GM01-50, the BS between Red Jungle Fowl and local village chicken increased to 72%. The results showed that the local village chicken is more closely related to Red Jungle Fowl than to broiler in the genetic distance. On the other hand, broiler is 1% closer in genetic distance to local village chicken than to Red Jungle Fowl. The results also indicated that primers like OPA-7, 8 and 9 can be used as species specific DNA markers for these three species of chickens.

Download File

PDF (Abstract)
DNA fingerprinting of red jungle fowl, village chicken and broilers.pdf

Download (36kB) | Preview
Official URL or Download Paper: http://www.ajas.info/journal/view.php?number=19687

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.5713/ajas.2000.1040
Publisher: Asian-Australasian Association of Animal Production Societies
Keywords: Broiler; Genome; Jungle fowl; RAPD; Village chicken
Depositing User: Nabilah Mustapa
Date Deposited: 30 Dec 2016 06:03
Last Modified: 30 Dec 2016 06:03
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5713/ajas.2000.1040
URI: http://psasir.upm.edu.my/id/eprint/50062
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item