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Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines


Citation

Wong, Shew Fung and Reimann, Karin and Lai, Leslie C. (2001) Effect of transforming growth factor-β1, insulin-like growth factor-I and insulin-like growth factor-II on cell growth and oestrogen metabolism in human breast cancer cell lines. Pathology, 33 (4). pp. 454-459. ISSN 0031-3025; ESSN: 1465-3931

Abstract / Synopsis

Oestrogens play an important role in the development of breast cancer. Oestrone sulphate (E1S) acts as a huge reservoir of oestrogens in the breast and is converted to oestrone (E1) by oestrone sulphatase (E1STS). E1 is then reversibly converted to the potent oestrogen, oestradiol (E2) by oestradiol-17β hydroxysteroid dehydrogenase (E2DH). The aim of this study was to assess the effects of transforming growth factor-β1 (TGFβ1), insulin-like growth factor-I (IGF-I) and insulin-like growth factor-II (IGF-II) on cell growth, E1STS and E2DH activities in the MCF-7 and MDA-MB-231 human breast cancer cell lines. TGFβ1, IGF-I and IGF-II alone or in combination inhibited cell growth of both cell lines but no additive or synergistic effects were observed. The treatments significantly stimulated E1STS activity in the MCF-7 cell line, except for TGF1 alone and TGF1 and IGF-I in combination, where no effects were seen. Only TGF1 and IGF-II acted synergistically to stimulate E1STS activity in the MCF-7 cells. There was no significant effect on E1STS activity in the MDA-MB-231 cells with any of the treatments. In the MCF-7 cells, TGFβ1 and IGF-I, IGF-I and IGF-II, and TGFβ1, IGF-I and IGF-II acted synergistically to stimulate the reductive E2DH activity, while only TGFβ1, IGF-I and IGF-II synergistically stimulated the oxidative E2DH activity. There were no additive or synergistic effects on both oxidative and reductive E2DH activities in the MDA-MB-231 cells. In conclusion, TGFβ1, IGF-I and IGF-II may have effects on oestrogen metabolism, especially in the MCF-7 cell line where they stimulated the conversion of E1S to E1 and E1 to E2 and, thus, may have roles to play in the development of breast cancer.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1080/00313020120083179
Publisher: Royal College of Pathologists of Australasia
Keywords: Transforming growth factor-β1; Insulin-like growth factor; Oestrogen metabolism; Estrogen metabolism; Breast cancer
Depositing User: Nabilah Mustapa
Date Deposited: 30 Dec 2016 13:56
Last Modified: 30 Dec 2016 13:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/00313020120083179
URI: http://psasir.upm.edu.my/id/eprint/50002
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