Simple Search:

Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography


Citation

Tan, Bee Koon and Foo, Hooi Ling and Loh, Teck Chwen and Abdullah, Norhani and Idrus, Zulkifli (2005) Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography. Asian-Australasian Journal of Animal Sciences, 18 (12). pp. 1780-1785. ISSN 1011-2367; ESSN: 1976-5517

Abstract / Synopsis

Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC). The fraction collected was then confirmed as VLDL using 4% polyacrylamide gel electrophoresis and transmission electron microscopy (TEM). The particle size of VLDL is 46.8 8.6 nm. The VLDL fraction was then subfractionated and the apolipoprotein (apo) profile was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE). The CB and AK have almost similar types of apo in both subfractions 1 and 2. The AK showed the presence of apoAI, AIV, D and E whereas the CB had apo AIV, D, E and H. The apo AIV and apo E were present in both subfractions of AK and CB.


Download File

[img]
Preview
PDF (Abstract)
Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography.pdf

Download (36kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.5713/ajas.2005.1780
Publisher: Asian-Australasian Association of Animal Production Societies
Keywords: Apolipoprotein; Broiler; Crossbred village chicken; Fast protein liquid chromatography; Very low density lipoprotein
Depositing User: Nabilah Mustapa
Date Deposited: 30 Dec 2016 13:37
Last Modified: 30 Dec 2016 13:37
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5713/ajas.2005.1780
URI: http://psasir.upm.edu.my/id/eprint/49976
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item