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Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC


Citation

Abd Mutalib, Sahilah and Sadi, Tosiah and Radu, Son and Ooi, Wai Ling and Queen, C. Y. K. and Lim, Jeffrey Seng Heng and Chua, S. C. and Mohd Tamin, Mohd Senawi (2005) Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC. Journal of Tropical Agriculture and Food Science, 33 (1). pp. 73-82. ISSN 1394-9829; ESSN: 2289-9650

Abstract / Synopsis

The epidemiological characteristics of Ralstonia solanacearum isolated from tomato plants in Sarawak and Selangor were studied. The epidemiological analysis of the strains was carried out through antibiotic resistant pattern, plasmid profiles, randomly amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) methods. Six strains were susceptible to all antibiotics tested, whereas the 10 strains that were resistant to one or more antibiotics were grouped into six antibiotic resistance patterns. Small single plasmid of 7.2 Mda and 9.2 Mda were detected among the nine plasmid containing strains, enabling them to be grouped into only two plasmid patterns. In the polymerase chain reaction, based methods using RAPD and ERIC, two strains were untypable by RAPD, whereas all were typable by ERIC. In this study, a wide diversity of R. solanacearum strains was examined. ERIC analysis demonstrated the clonal relationship between isolates from tomato plants in Sarawak and Selangor. The existence of similar R. solanacearum strains in tomato plants from two very distant locations should be considered if tomato strain fingerprint results were to be used to help trace the vehicles for transmission.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Biotechnology
Publisher: Malaysian Agricultural Research and Development Institute (MARDI)
Keywords: Ralstonia solanacearum; Antibiotic susceptibility; Plasmid profiles; RAPD-PCR; ERIC-PCR
Depositing User: Nabilah Mustapa
Date Deposited: 29 Dec 2016 17:58
Last Modified: 29 Dec 2016 17:58
URI: http://psasir.upm.edu.my/id/eprint/48936
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