Citation
Mohd Danial, Azlina and Long, Kamariah and Mohd Ghazali, Hasanah
(2004)
Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus.
Journal of Tropical Agriculture and Food Science, 32 (2).
pp. 187-195.
ISSN 1394-9829; ESSN: 2289-9650
Abstract
Specific activity of lipase extracted from the mycelium of Aspergillus flavus, obtained from 3-day-old culture, increased nearly two times higher than from its culture supernatant. Different types of parameters were tested to optimise the extraction of mycelium-bound lipase. Highest specific activity of A. flavus lipase was obtained at 30 °C for 30 min with the agitation rate of 200 rev/min. Tris-HCl buffer (0.05 M) at pH 8.2 was the best extractant followed by Triton X-100 (0.1%), EDTA (0.01 M), NaCl (1 M) and Tween-80 (0.01%). The optimised conditions developed for the extraction of mycelium-bound lipase improved the purity of this lipase by 2.9 times higher than the control.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Food Science and Biotechnology |
| Publisher: | Malaysian Agricultural Research and Development Institute (MARDI) |
| Keywords: | Mycelium-bound lipase; Specific activity; Extracted lipase; Free lipase |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 29 Dec 2016 09:58 |
| Last Modified: | 29 Dec 2016 09:58 |
| URI: | http://psasir.upm.edu.my/id/eprint/48935 |
| Statistic Details: | View Download Statistic |
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