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Effects of cattle oocyte quality on pronuclear formation and subsequent embryo development


Citation

Bidin, Habsah and Kamaruddin, Musaddin and Ishak, Mohd Rafi (2009) Effects of cattle oocyte quality on pronuclear formation and subsequent embryo development. Journal of Tropical Agriculture and Food Science, 37 (2). pp. 271-277. ISSN 1394-9829; ESSN: 2289-9650

Abstract / Synopsis

The effect of oocyte quality on the pronuclear (PN) formation and subsequent development of embryos in local cattle was studied. Cattle oocytes matured and fertilized in vitro were unable to acquire developmental competence unless matured with intact cumulus cells. The presence of cumulus cells promoted normal fertilization with proper pronuclear (2PN) formation. In the present study, the characteristics and quality of cumulus-oocyte-complexes influenced the pronuclear formation but not subsequent cleavage and blastocyst formation. The oocytes from Grade A, Grade B and Grade B' were capable of fertilization and development under in vitro conditions. Fertilization rates were significantly different (p <0.05) among the three grades of oocytes. Grade A had the highest fertilization rates (55.0%) followed by Grade B (44.0%) and Grade B' (30.2%). Incidence of polyspermy (>2PN) was 18.8% in Grade B' oocytes but none in Grades A and B oocytes. The mean percentages of cleavage and blastocyst rates were not significant (p >0.05) in Grades A, B and B' (71.6, 74.9 and 73.6%, respectively, and 10.5, 10.4 and 7.4%, respectively). In conclusion, this study indicated that the compactness of cumulus cells surrounding the oocytes influenced the pronuclear formation of cattle oocytes but not cleavage and blastocyst rates.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Educational Studies
Publisher: Malaysian Agricultural Research and Development Institute (MARDI)
Keywords: Pronuclear formation; Blastocyst; Cumulus-oocyte complex; In vitro fertilization; Cleavage; Cattle embryo
Depositing User: Nabilah Mustapa
Date Deposited: 29 Dec 2016 17:55
Last Modified: 29 Dec 2016 17:55
URI: http://psasir.upm.edu.my/id/eprint/48902
Statistic Details: View Download Statistic

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