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Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines


Citation

Rajab, Nor Fadilah and Abd. Hamid, Zariyantey and Hassan, Hunaizah and Ali, Abdul Manaf and Din, Laily and Inayat Hussain, Salmaan Hussain (2005) Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines. Environmental Mutagen Research, 27 (3). pp. 161-164. ISSN 0910-0865; ESSN: 1880-7054

Abstract / Synopsis

The cytotoxic and genotoxic effects of goniothalamin, a plant styryllactone, were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and the Alkaline Comet assay respectively in human leukemic cell lines. Following 72 h of treatment, the IC50 values of goniothalamin in human HL-60 promyelocytic leukemia cells and CEM-SS T-lymphoblastic cells were 4.5 μg/mL and 2.4 μg/mL respectively. The genotoxicity of goniothalamin in both HL-60 and CEM-SS cells was detected as early as 2 h following treatment at IC10 and IC25 concentrations. However, pretreatment with the antioxidant N-acetyl-cysteine (NAC) at 1 mM for 30 minutes did not abrogate genotoxicity of this compound. This result suggests that primary induction of DNA damage by goniothalamin may not involve oxidative damage. In conclusion, our results demonstrate genotoxic damage induced by goniothalamin in leukemic cells. Further studies are needed to ascertain the mode of action of goniothalamin in inducing DNA damage.


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Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
DOI Number: https://doi.org/10.3123/jems.27.161
Publisher: The Japanese Environmental Mutagen Society
Keywords: Cytotoxicity; Genotoxicity; Alkaline Comet Assay; Leukemic cell lines
Depositing User: Nabilah Mustapa
Date Deposited: 10 Oct 2016 11:47
Last Modified: 10 Oct 2016 11:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3123/jems.27.161
URI: http://psasir.upm.edu.my/id/eprint/48591
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