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Tamoxifen-loaded solid lipid nanoparticles-induced apoptosis in breast cancer cell lines


Citation

Abbasalipourkabirreh, Roghayeh and Salehzadeh, Aref and Abdullah, Rasedee (2016) Tamoxifen-loaded solid lipid nanoparticles-induced apoptosis in breast cancer cell lines. Journal of Experimental Nanoscience, 11 (3). pp. 161-174. ISSN 1745-8080; ESSN: 1745-8099

Abstract / Synopsis

An in vitro study was conducted to determine the apoptosis induced by tamoxifen (TAM) and TAM-loaded solid lipid nanoparticles (SLNs) in breast cancer cell lines, MCF-7 and MDA-MB231 cells. The effect of free drug and drug-loaded SLN on the cell lines was characterised by cell morphology and cell cycle distribution using phase contrast microscopy, nuclear morphology and flow cytometry, respectively. The results showed that TAM-loaded SLNs have an equally efficient cytotoxic activity against MCF-7 and MDA-MB231 cells, compared to free TAM, and the half maximal inhibitory concentration (IC50) of TAM-loaded SLNs was generally lower than that of free TAM. In the presence of TAM and TAM-loaded SLN, the viability of the both cells diminishes and the cancer cells lose their normal morphological characteristics, detaches, aggregates and later develops apoptotic bodies. Flow cytometry analysis showed that TAM-loaded SLN like the free TAM caused a dose- and time-dependent apoptosis without cell cycle arrest of human breast cancer cells. Therefore, TAM-loaded SLN has great potential in human medicine for the treatment of breast cancers.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1080/17458080.2015.1038660
Publisher: Taylor & Francis
Keywords: Apoptosis; Breast cancer cell lines; Solid lipid nanoparticles; Tamoxifen
Depositing User: Nabilah Mustapa
Date Deposited: 20 May 2016 10:56
Last Modified: 20 May 2016 10:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/17458080.2015.1038660
URI: http://psasir.upm.edu.my/id/eprint/47511
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