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Confirmation of non N-glycan linked mannose glycosylation in chitinase 42 kDa secreted by Trichoderma harzianum BIO10671


Mustafa, Muskhazli and Qamaruz Zaman, Faridah and Ramli, Salfarina and Tohfah, Nor Farizan and Ithnin, Nalisha and Wallis, Gregg L. F. (2008) Confirmation of non N-glycan linked mannose glycosylation in chitinase 42 kDa secreted by Trichoderma harzianum BIO10671. Asian Journal of Biochemistry, 3 (4). pp. 235-242. ISSN 1815-9923; ESSN: 1815-9931


Citinase 42 kDa produced by Trichoderma harzianum has been proven as a prime compund to be excreted onto the hyphae of the pathogen causing localised cell wall lysis at the point of interaction. This finally initiate the process of the host cell becomes empty of cytoplasm, disintegrates and shows a rapid collapse. This study investigates the existence of N-glyan linked mannose in chitinase 42 kDa from T.harzianum BIO10671 was initially purified using anion exchange chromatography prior to a series of experiments such as immunoblotting against the chitinase 42 kDa antibody, lectin staining for detecting any terminal linked mannose and galactofuranose detection to determine the presence of galactofuranose components in glycoproteins. The enzyme purification varvested about 12-fold of chitinase 42 kDa after overnight incubation in chitinase 42 kDa antibody suggesting taht the gene for chitinase 42 kDa was greatly expressed in this strain.There are no intervation of galactofuranose on any of the terminal mannose in chitinase 42 kDa as shown by negative results on samples treated with or without endoglycosidase-H and lectin staining. Therefore, it can be concluded that glycosylation occured in the chitinase 42 kDa from T.harianum 42 kDa was not in the form of N-glycan linked mannose as aspected.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: https://doi.org/10.3923/ajb.2008.235.242
Publisher: Academic Journals
Keywords: Trichoderma harzianum; Chitinase; Glycosylation; N-glycan linked; Mannose
Depositing User: Najwani Amir Sariffudin
Date Deposited: 25 Mar 2010 11:19
Last Modified: 27 Oct 2017 03:50
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ajb.2008.235.242
URI: http://psasir.upm.edu.my/id/eprint/4698
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