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Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda citrifolia L.)


Citation

Mohd Zin, Zamzahaila and Abdul Hamid, Azizah and Osman, Azizah and Saari, Nazamid (2006) Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda citrifolia L.). Food Chemistry, 94 (2). pp. 169-178. ISSN 0308-8146

Abstract / Synopsis

Crude extracts of root, leaf and fruit of Morinda citrifolia were fractionated on a Sephadex LH-20 column with ethanol as eluate. Based on UV absorption intensity of phenolic compound (725 mm) the Sephadex LH-20 column was able to separate fruit, leaf and root extracts into six, five and five fractions, respectively. The results showed that all the fractions tested exhibited considerably high antioxidative activity in the ferric thiocyanate assay and thiobarbituric acid test and the activities of some of the fractions were as good as those of either tocopherol or BHT. The fractions from different parts of the plants were found to contain different amounts of total phenolic compounds, which, interestingly, do not correspond to the antioxidative activity measured. This is probably due to the presence of different phenolics in the samples, with different antioxidative activities which involves various mechanisms inhibiting the oxidation process. The study suggested that root, leaf and fruit of M. citrifolia might contribute significantly to exogenous antioxidant which is crucial in combating oxidative stress.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1016/j.foodchem.2004.08.048
Publisher: Elsevier
Keywords: Morinda citrifolia, Antioxidative activity, Chromatographic fractions
Depositing User: Samsida Samsudin
Date Deposited: 17 May 2010 04:29
Last Modified: 26 Oct 2015 03:08
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodchem.2004.08.048
URI: http://psasir.upm.edu.my/id/eprint/4672
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