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Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction


Citation

Asad, H. A. and Meah, M. B. and Begum, S. N. and Khalil, M. I. and Yusop, Mohd Rafii and Latif, M. A. (2015) Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction. Genetics and Molecular Research, 14 (4). pp. 17007-17018. ISSN 1676-5680

Abstract

Disease susceptibility and genetic variability in 10 eggplant genotypes were studied after inoculating Phomopsis vexans under confined field conditions. Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation and relationships among eggplant genotypes. The disease index of leaves ranged 0.208-13.79%, while fruit infection ranged 2.15-42.76%. Two varieties, Dohazari G and Laffa S, were found to be susceptible, 6 were moderately resistant, 1 was moderately susceptible, and BAU Begun-1 was resistant to P. vexans. Amplification of genomic DNA by using 3 RAPD primers produced 20 bands: 14 (70%) were polymorphic and 6 (30%) were monomorphic. The highest intra-variety similarity indices values were found in ISD 006, Ishurdi L, Jessore L, and BAU Begun-1 (100%), while the lowest was in Dohazari G (90%). The lowest genetic distance (0.0513) and the highest genetic identity (0.9500) were observed between the ISD 006 and Ishurdi L combinations. A comparatively higher genetic distance (0.3724) and the lowest genetic identity (0.6891) were observed between the ISD 006 and Dohazari G combinations. A dendogram was constructed based on Nei’s genetic distance, which produced 2 main clusters of the genotypes - Cluster I: ISD 006, Ishurdi L, Marich begun L, BAU Begun-1, Marich begun S, and Chega and Cluster 2: Laffa S, Dohazari G, Jessore L, and Singhnath. Genetic variation and its relationship with disease susceptibility were assessed using RAPD markers, to develop disease-resistant varieties and improve eggplant crops.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.4238/2015.December.16.1
Publisher: Fundacao de Pesquisas Cientificas de Ribeirao Preto
Keywords: Eggplant; Genetic variation; Phomopsis blight; Resistance; Random amplified polymorphic DNA
Depositing User: Nida Hidayati Ghazali
Date Deposited: 27 Feb 2018 08:21
Last Modified: 27 Feb 2018 08:21
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4238/2015.December.16.1
URI: http://psasir.upm.edu.my/id/eprint/46413
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