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Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity


E., Sabina and I. S., M. Zaidul and Ghafoor, Kashif and M. Jaffri, J. and F., Sahena and Babiker, E. E. and Perumal, V. and Mirhosseini, Hamed and Mehrnoush, Amid and A., Khatib (2016) Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity. Journal of Food Biochemistry, 40 (2). pp. 201-210. ISSN 0145-8884; ESSN: 1745-4514


Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α-glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa. Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room temperature solvent extraction, were evaluated for in vitro α-glucosidase activity inhibition. The compounds were identified by using gas chromatography-mass spectrometry (GC-MS) according to their similarity index of >70%, which might be responsible for α-glucosidase inhibitory activity. The methanol extract of the fruit flesh had the highest yield (25.6 ± 0.5%), whereas the n-hexane extract of the stem is more effective against α-glucosidase activity (IC50 0.8 ± 0.1 μg/mL). The fruit flesh (IC501.3 ± 0.2 μg/mL) and leaves (IC501.6 ± 0.6 μg/mL) had also well effectively. The identified metabolites are predominantly phenolics, carbohydrates, triterpenes and organic acids, such as D-fructose, squalene, α-linolenic acid and α-D-glucopyranoside. In-depth chemical profiling using GC-MS was performed for the first time for this plant to assess the likely compounds present in the extract that could be associated with anti-hyperglycemic activity. Of the three parts tested, every part indicates the potential α-glucosidase inhibitory activity and hexane extract of stem showed more inhibitory activity among all extracts. Thus, P. macrocarpa can attenuate hyperglycemia by potently inhibiting carbohydrate hydrolyzing enzymes, making it a viable plant as a source of natural compounds for the management of type 2 diabetes mellitus.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1111/jfbc.12212
Publisher: Wiley-Blackwell Publishing
Keywords: Phaleria macrocarpa; Herbal plant; α-glucosidase inhibitory activity; Malaysia
Depositing User: Ms. Ainur Aqidah Hamzah
Date Deposited: 16 Jun 2022 08:23
Last Modified: 16 Jun 2022 08:23
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/jfbc.12212
URI: http://psasir.upm.edu.my/id/eprint/46232
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