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Rat full term amniotic fluid harbors highly potent stem cells


Hoo, Mun-Fun and Ferdaos, Nurfarhana and Hamzah, Siti Nurusaadah and Ridzuan, Noridzzaida and Hisham, Nurul Afiqah and Abdullah, Syahril and Ramasamy, Rajesh and Cheah, Pike See and Karuppiah, Thilakavathy and Yazid, Mohd Nazri and Nordin, Norshariza (2015) Rat full term amniotic fluid harbors highly potent stem cells. Research in Veterinary Science, 102. pp. 89-99. ISSN 0034-5288


Amniotic fluid stem cells (AFSCs) are commonly isolated from mid-term amniotic fluid (AF) of animals and human collected via an invasive technique, amniocentesis. Alternatively, AFSCs could be collected at full-term. However, it is unclear whether AFSCs are present in the AF at full term. Here, we aimed to isolate and characterize stem cells isolated from AF of full term pregnant rats. Three stem cell lines have been established following immuno-selection against the stem cell marker, c-kit. Two of the new lines expressed multiple markers of pluripotency until more than passage 90. Further, they spontaneously differentiated into derivatives of the three primary germ layers through the formation of good quality embryoid bodies (EBs), and can be directly differentiated into neural lineage. Their strong stemness and potent neurogenic properties highlight the presence of highly potent stem cells in AF of full-term pregnancies, which could serve as a potential source of stem cells for regenerative medicine.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.rvsc.2015.07.010
Publisher: Elsevier
Keywords: Full term amniotic fluid; Amniotic fluid stem cells (AFSCs); Potentially pluripotent; Broad multipotent stem cells; Pluripotency test; Embryoid bodies; Neurogenic potential
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 23 Mar 2022 04:29
Last Modified: 23 Mar 2022 04:29
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.rvsc.2015.07.010
URI: http://psasir.upm.edu.my/id/eprint/46081
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