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Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus


Citation

Sabullah, Mohd Khalizan and Abd. Shukor, Mohd Yunus and Shamaan, Nor Aripin and Khalid, Ariff and Ganzau, Azlan Jualang and Sulaiman, Mohd Rosni and Jirangon, Hussain and Ahmad, Siti Aqlima (2015) Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus. International Journal of Agriculture and Biology, 17 (5). pp. 1025-1030. ISSN 1560-8530; ESSN: 1814-9596

Abstract

The purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow rate at 0.2 mL/min. Non-denaturing electrophoresis condition was employed and the single band native form of ChE was detected at 66.267 kDa after being stained with commasie brilliant blue. ChE detection was performed using gel filtration; ZORBAX column attached to the HPLC with the flow rate of 1 mL/min. Only a single peak was detected at the retention time of 3.720. From the assay evaluation, the final purified ChE procedure displayed the highest sensitivity of detecting the anticholinesterase namely mercury, copper, malaoxon and carbofuran compared to the impure ChE and the results were further discussed in detail to the potential application of ChE from P. javanicus as a biomarker for those toxicants.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.17957/IJAB/15.0012
Publisher: Friends Science Publishers
Keywords: Purification; Cholinesterase; Puntius Javanicus; Anticholinesterase
Depositing User: Nida Hidayati Ghazali
Date Deposited: 08 May 2018 01:33
Last Modified: 08 May 2018 01:33
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.17957/IJAB/15.0012
URI: http://psasir.upm.edu.my/id/eprint/46049
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