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Pluripotency maintenance of amniotic fluid-derived stem cells cultured on biomaterials


Citation

Wang, Pin Yu and Lee, Henry Hsin Chung and Higuchi, Akon and Ling, Qing Dong and Lin, Hong Ren and Li, Hsin Fen and Kumar, S. Suresh and Chang, Yung and Alarfaj, Abdullah A. and Munusamy, Murugan A. and Chen, Da Chung and Hsu, Shih Tien and Wang, Han Chow and Hsiao, Hung Yi and Wu, Gwo Jang (2015) Pluripotency maintenance of amniotic fluid-derived stem cells cultured on biomaterials. Journal of Materials Chemistry B, 3 (18). pp. 3858-3869. ISSN 2050-750X; ESSN: 2050-7518

Abstract

The stem cell fates of pluripotency and differentiation are regulated by not only soluble biological cues but also insoluble biochemical cues (i.e., extracellular matrix (ECM)) and the physical cues of cell culture biomaterials (i.e., elasticity). We investigated the maintenance of pluripotency and the differentiation lineages of human amniotic fluid-derived stem cells (hAFSCs) cultured on poly(vinyl alcohol-co-itaconic acid) (PVA) hydrogels grafted with several types of ECM and corresponding oligopeptides in expansion medium. hAFSCs cultured on soft PVA hydrogels (12.2 kPa) that were grafted with oligopeptides derived from fibronectin and vitronectin showed high pluripotency, which was evaluated by Oct4, Sox2 and Nanog expression. The hAFSCs grown on soft PVA hydrogels (12.2 kPa) grafted with each oligopeptide showed higher pluripotency, as assessed by Oct4 and Nanog expression, than hAFSCs grown on stiff PVA hydrogels (25.3 kPa) grafted with the same oligopeptides and a much higher pluripotency than those grown on rigid tissue-culture polystyrene dishes. Soft biomaterials appeared to be adequate to maintain the pluripotency of hAFSCs. Surprisingly, hAFSCs that showed higher pluripotency on PVA hydrogels grafted with oligopeptides derived from fibronectin and vitronectin also expressed higher levels of early differentiation markers for three germ layers in expansion medium. This result suggests that hAFSCs are heterogeneous and that this population contains highly pluripotent stem cells and stem cells that can be easily differentiated.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1039/c5tb00447k
Publisher: Royal Society of Chemistry
Keywords: Stem cell; Poly(vinyl alcohol-co-itaconic acid); PVA hydrogels
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 30 Apr 2021 08:24
Last Modified: 30 Apr 2021 08:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1039/c5tb00447k
URI: http://psasir.upm.edu.my/id/eprint/45885
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