UPM Institutional Repository

Nordamnacanthal potentiates the cytotoxic effects of tamoxifen in human breast cancer cells


Mohamed Alitheen, Noorjahan Banu and Nik Abd Rahman, Nik Mohd Afizan and Ho, Chai Ling and Subramani, Tamilselvan and Osman, Che Puteh and Ismail, Nor Hadiani and Swee, Keong Yeap and Wan, Yang Ho (2015) Nordamnacanthal potentiates the cytotoxic effects of tamoxifen in human breast cancer cells. Oncology Letters, 9 (1). pp. 335-340. ISSN 1792-1082; ESSN: 1792-1074


Tamoxifen (TAM) is the mainline drug treatment for breast cancer, despite its side effects and the development of resistance. As an alternative approach, in the present study a novel combination therapy was established through combining TAM with nordamnacanthal (NDAM) in order to investigate the additive effect of these drugs in MCF-7 human breast cancer cells. A significant dose-dependent reduction in cell viability and an increase in apoptosis were observed in the MCF-7 cells cotreated with TAM and NDAM compared with the untreated control cells or the cells treated with TAM and NDAM alone (P<0.05). The cytotoxic influence of the combination of TAM and NDAM was found to be two-fold that of the individual agents. Annexin V/propidium iodide double-staining revealed the typical nuclear features of apoptosis. Furthermore, an increase in the proportion of apoptotic, Annexin V-positive cells was observed with the combination therapy. Moreover, this apoptotic induction was associated with a collapse of the mitochondrial membrane potential and the generation of reactive oxygen species. To the best of our knowledge, the findings of the present study are the first to suggest that combining TAM with NDAM may be a potential combination therapy for the treatment of breast cancer and may have the potential to minimize or eliminate the side effects associated with high doses of TAM.

Download File

[img] Text (Abstract)

Download (6kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.3892/ol.2014.2697
Publisher: Spandidos Publications
Keywords: Tamoxifen; Nordamnacanthal; MCF-7; Breast cancer; Combination treatment; Apoptosis
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 23 Jan 2021 22:07
Last Modified: 23 Jan 2021 22:07
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3892/ol.2014.2697
URI: http://psasir.upm.edu.my/id/eprint/45610
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item