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Mechanisms of gastroprotection of methanol extract of Melastoma malabathricum leaves


Zakaria, Zainul Amiruddin and Mohtarrudin, Norhafizah and Teh, Lay Kek and Salleh, Mohd Zaki and Balan, Tavamani and Mamat, Siti Syariah (2015) Mechanisms of gastroprotection of methanol extract of Melastoma malabathricum leaves. BMC Complimentary and Alternative Medicine, 15 (135). pp. 1-15. ISSN 1472-6882


Background: Melastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the gastroprotective mechanisms of methanol extract of M. malabathricum leaves (MEMM) in rats. Methods: The extract's mechanisms of gastroprotection (50, 250, 500 mg/kg) were studied using the pylorus-ligation in rat model wherein volume, pH, free and total acidity of gastric juice, and gastric wall mucus content were determined. The involvement of endogenous nitric oxide (NO) and sulfhydryl (SH) compounds in the gastroprotective effect of MEMM were also measured. MEMM was subjected to the antioxidant, anti-inflammatory and phytochemical analysis and HPLC profiling. Results: MEMM contained various phyto-constituents with quercitrin being identified as part of them. MEMM and quercitrin: i) significantly (p < 0.05) reduced the volume and acidity of gastric juice while increasing the pH and gastric wall mucus content.; ii) significantly (p < 0.05) increased the level of SOD, GTP and GTR while significantly (p < 0.05) reduced the level of CAT, MPO and TBARS activities.; iii) exerted gastroprotective activity when assessed using the ethanol-induced gastric ulcer assay, which was reversed by NG-nitro-l-arginine methyl esters (L-NAME; an inhibitor of NO synthase) and N-ethylmaleimide (NEM; a sulfhydryl (SH) blocker). MEMM inhibited the lipoxygenase (LOX) and xanthine oxidase (XO) activities with the highest affinity for the former while quercitrin showed high affinity for XO activity. Conclusions: MEMM exhibited a gastroprotective activity due partly to the presence of quercitrin, its antioxidant and anti-inflammatory activities, and via the modulation of NO and SH groups.

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Official URL or Download Paper: https://pubmed.ncbi.nlm.nih.gov/25927982/

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Halal Products Research Institute
DOI Number: https://doi.org/10.1186/s12906-015-0638-z
Publisher: BioMed Central
Keywords: Melastoma malabathricum; Melastomaceae; Methanol extract; Gastroprotective mechanisms; Nitric oxide; Sulfhydryl group; Antioxidant; Anti-inflammatory
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 23 Jan 2021 22:18
Last Modified: 23 Jan 2021 22:18
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1186/s12906-015-0638-z
URI: http://psasir.upm.edu.my/id/eprint/45441
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