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In vivo antitumor and antimetastatic effects of flavokawain B in 4T1 breast cancer cell-challenged mice


Abu, Nadiah and Mohamed, Nurul Elyani and Swee, Keong Yeap and Kian, Lam Lim and Akhtar, Muhammad Nadeem and Zulfadli, Aimi Jamil and Beh, Boon Kee and Abdullah, Mohd Puad and Omar, Abdul Rahman and Alitheen, Noorjahan Banu (2015) In vivo antitumor and antimetastatic effects of flavokawain B in 4T1 breast cancer cell-challenged mice. Drug Design, Development and Therapy, 9. pp. 1401-1407. ISSN 1177-8881


Flavokawain B (FKB) is a naturally occurring chalcone that can be isolated through the root extracts of the kava-kava plant (Piper methysticum). It can also be synthesized chemically to increase the yield. This compound is a promising candidate as a biological agent, as it is reported to be involved in a wide range of biological activities. Furthermore, FKB was reported to have antitumorigenic effects in several cancer cell lines in vitro. However, the in vivo antitumor effects of FKB have not been reported on yet. Breast cancer is one of the major causes of cancer-related deaths in the world today. Any potential treatment should not only impede the growth of the tumor, but also modulate the immune system efficiently and inhibit the formation of secondary tumors. As presented in our study, FKB induced apoptosis in 4T1 tumors in vivo, as evidenced by the terminal deoxynucleotidyl transferase dUTP nick end labeling and hematoxylin and eosin staining of the tumor. FKB also regulated the immune system by increasing both helper and cytolytic T-cell and natural killer cell populations. In addition, FKB also enhanced the levels of interleukin 2 and interferon gamma but suppressed interleukin 1B. Apart from that, FKB was also found to inhibit metastasis, as evaluated by clonogenic assay, bone marrow smearing assay, real-time polymerase chain reaction, western blot, and proteome profiler analysis. All in all, FKB may serve as a promising anticancer agent, especially in treating breast cancer.

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Official URL or Download Paper: https://pubmed.ncbi.nlm.nih.gov/25834398/

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.2147/DDDT.S67976
Publisher: Dove Medical Press
Keywords: Favokawain B; Kava-kava; 4T1; Cancer; Metastasis
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 16 Apr 2021 02:52
Last Modified: 16 Apr 2021 02:52
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2147/DDDT.S67976
URI: http://psasir.upm.edu.my/id/eprint/45115
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