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Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment


Citation

Ude, Chinedu C. and Ng, Min Hwei and Chen, Hui Cheng and Htwe, Ohnmar and Amaramalar, Naicker Sylva and Hassan, Md. Shariful and Djordjevic, Ivan and Abdul Rani, Rizal and Ahmad, Johan and Mohammad Yahya, Norhamdan and Saim, Aminuddin and Idrus, Ruszymah (2015) Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment. Osteoarthritis and Cartilage, 23 (8). pp. 1294-1306. ISSN 1063-4584; ESSN:1522-9653

Abstract

Objectives: Our previous studies on osteoarthritis (OA) revealed positive outcome after chondrogenically induced cells treatment. Presently, the functional improvements of these treated OA knee joints were quantified followed by evaluation of the mechanical properties of the engineered cartilages. Methods: Baseline electromyogram (EMGs) were conducted at week 0 (pre-OA), on the locomotory muscles of nine un-castrated male sheep (Siamese long tail cross) divided into controls, adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs), before OA inductions. Subsequent recordings were performed at week 7 and week 31 which were post-OA and post-treatments. Afterwards, the compression tests of the regenerated cartilage were performed. Results: Post-treatment EMG analysis revealed that the control sheep retained significant reductions in amplitudes at the right medial gluteus, vastus lateralis and bicep femoris, whereas BMSCs and ADSCs samples had no further significant reductions (P < 0.05). Grossly and histologically, the treated knee joints demonstrated the presence of regenerated neo cartilages evidenced by the fluorescence of PKH26 tracker. Based on the International Cartilage Repair Society scores (ICRS), they had significantly lower grades than the controls (P < 0.05). The compression moduli of the native cartilages and the engineered cartilages differed significantly at the tibia plateau, patella femoral groove and the patella; whereas at the medial femoral condyle, they had similar moduli of 0.69 MPa and 0.40-0.64 MPa respectively. Their compression strengths at all four regions were within ±10 MPa. Conclusion: The tissue engineered cartilages provided evidence of functional recoveries associated to the structural regenerations, and their mechanical properties were comparable with the native cartilage.


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Official URL or Download Paper: https://pubmed.ncbi.nlm.nih.gov/25887366/

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1016/j.joca.2015.04.003
Publisher: Elsevier
Keywords: Cartilage; Cell therapy; Function; Osteoarthritis; Regeneration
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 18 Apr 2021 00:37
Last Modified: 18 Apr 2021 00:37
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.joca.2015.04.003
URI: http://psasir.upm.edu.my/id/eprint/45078
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